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Measurement of telomere DNA content by dot blot analysis. Oxford University Press is a department of the University of Oxford. It furthers the University's objective of excellence in research, scholarship, and education by publishing worldwide.
Sign In or Create an Account. Sign In. Advanced Search. Search Menu. Article Navigation. Close mobile search navigation Article Navigation. Volume Article Contents Abstract. M ethods. R esults. D iscussion. F unding. C onflict of I nterest. R eferences. Elbers , Clara C. Oxford Academic. Melissa E. Masayuki Kimura. Steven R. Mike A. Anne B. Vicki Park. Jason L. Gregory J. Sarah A. Tamara B. Harris , Tamara B. Abraham Aviv. Cite Cite Clara C. Select Format Select format. Permissions Icon Permissions.
Table 1. Age y Open in new tab. Open in new tab Download slide. Table 2. Beta SD. Google Scholar PubMed. Google Scholar Crossref. Search ADS. Diez Roux. All rights reserved. For permissions, please e-mail: journals. Issue Section:. Download all slides. View Metrics. Email alerts Article activity alert. New issue alert. Building upon this technology, radiolabelled nucleotides were replaced with fluorescently labeled ones allowing for fluorometric-based detection.
This increased sequencing speed ultimately leading to what are generally considered first generation DNA sequencing machines 9. Today, similar technologies, sometimes referred to as sequence DNA as it synthesized by electronically recording fluorescence activity as fluorescently labeled nucleotides are incorporated, a method called pyrosequencing. The drive for faster, higher throughput and less expensive sequencing fuels a sequencing revolution that still prevails. Sequencing whole genomes or profiling transcriptomes RNAseq require sequencing of many samples at once for later comparison.
These next-generation sequencing techniques do not typically rely on Sanger sequencing but instead use methods like pyrosequencing that are parallelized to sequence thousands of reads at the same time. Other applications of sequencing, such as confirming the results of a cloning experiment, require only one or a few sequencing reactions and may use Sanger sequencing.
Southern blotting, restriction mapping and sequencing gels are classic techniques that allowed for many seminal scientific discoveries 5 , 8. While newer techniques have largely replaced these, these techniques still have useful applications and have informed many current techniques. For example, restriction mapping led to recombinant DNA technologies that are now commonplace in laboratories.
As scientific technologies rapidly progress, some common lab techniques we use now may soon become obsolete. In the sequencing world, one up and coming innovation is nanopore-based DNA sequencing which reads the nucleotides of a DNA strand as it slips through a tiny pore by measuring differences in ion flow caused by the different electrochemical properties of nucleotides 8.
Compared to older technologies nanopore-based DNA sequencing machines are relatively small meaning that DNA sequencing could move from sequencing cores to individual labs and even out into the field. Current versions are already commercially available although they are reported to have high error rates The future of sequencing should bring efficient, low cost methods for sequencing large amounts of DNA quickly and accurately. We're sure to see many improvements in a variety of biological technologies as both time and research progress.
These will bring faster, higher throughput, and potentially more accurate techniques to the hands of biologists throughout the world. Let us know your favorite "Historical Lab Technique" in the comments section below! Krissy Lyon is a PhD candidate in Neuroscience at Harvard University currently studying the serotonergic system and behavior.
Southern, Edwin Mellor. Chang, Judy C. Roberts, Richard J. Danna, Kathleen, and Daniel Nathans. Heather, James M. Hunkapiller, Tom, et al. Feng, Yanxiao, et al.
Add Comment. Addgene is a nonprofit plasmid repository. We archive and distribute high quality plasmids from your colleagues. Restriction mapping and modern sequencing Restriction mapping informs on DNA sequence through the use of restriction enzymes that cut DNA at specific sites 5. Sequencing gels and next-generation sequencing techniques Introduced in , Sanger sequencing, also known as chain-terminating or dideoxy sequencing, was a major breakthrough for determining the order of nucleotides in a string of DNA 7.
Looking to the future Southern blotting, restriction mapping and sequencing gels are classic techniques that allowed for many seminal scientific discoveries 5 , 8. Many thanks to our guest blogger Krissy Lyon! References 1. Sharing science just got easier Subscribe to our blog.
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